Composite
Part:BBa_K5349006:Design
Designed by: Tomoharu Sakata Group: iGEM24_BotchanLab-Tokyo (2024-09-26)
pNorV-sbm operon-sfGFP-norR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3147
Illegal BglII site found at 5418
Illegal BamHI site found at 1251
Illegal BamHI site found at 1447
Illegal BamHI site found at 3584
Illegal XhoI site found at 6122 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 781
Illegal SapI.rc site found at 4043
Design Notes
For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Integrated DNA Technologies' DNA synthesis service.
Source
- pNorV:
- RBS:
- Sbm operon:
- scpA-argK:
- scpB:
- scpC:
- sfGFP:
- norR:
- terminator: