Composite

Part:BBa_K5349006:Design

Designed by: Tomoharu Sakata   Group: iGEM24_BotchanLab-Tokyo   (2024-09-26)


pNorV-sbm operon-sfGFP-norR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3147
    Illegal BglII site found at 5418
    Illegal BamHI site found at 1251
    Illegal BamHI site found at 1447
    Illegal BamHI site found at 3584
    Illegal XhoI site found at 6122
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 781
    Illegal SapI.rc site found at 4043


Design Notes

For cloning, overlap sequences were designed for each fragment, and the assembly was performed using NEBuilder. Each fragment was synthesized using Integrated DNA Technologies' DNA synthesis service.


Source

  • pNorV:
  • RBS:
  • Sbm operon:
    • scpA-argK:
    • scpB:
    • scpC:
  • sfGFP:
  • norR:
  • terminator:

References